FastQCFastQC Report
Mon 3 Apr 2023
EGAF00004455724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32989134
Sequences flagged as poor quality0
Sequence length50-120
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAAC2727950.8269237986059288No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG1596310.48388963469001645No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA677030.2052281821038406No Hit
GATATTCGCAAGAACACCAGTGGCGAAGGCGGCTCACTGGCCCGATACTG643630.19510363624580143No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA623550.1890167835263575No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA614330.18622192386135386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT443830.13453823916687235Clontech Universal Primer Mix Long (96% over 26bp)
TACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGATCTGATGTGGAGG331090.1003633499442574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)