FastQCFastQC Report
Sat 1 Apr 2023
EGAF00004455727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20136689
Sequences flagged as poor quality0
Sequence length50-120
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA934170.4639144002273661No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA654770.325162691840749No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA475670.23622056237745936No Hit
GGCTTGCCGATCTTGTTCCCCCAGTAGCCTCTGCGCACGGGGACGATGGA432410.2147373880581857No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC430120.2136001603838645No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA417080.20712441851786062No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC350780.1741994426193899No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG344390.1710261304626595No Hit
AGTCAGTGGTGGCCTGAGCGGGGATGGCGACCGAGAAGGAGCTGATTGCT314490.1561776119202119No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT299880.1489221986792367No Hit
AGCCCTCTCGCCTCCCTGCCTCACCGGGCAGGCCCTGGCTGGCGCACGTG282730.14040540627111042No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA261720.12997171481369157No Hit
TCCCGGTAACGCTTCAGCACCTGGGCCAGGTCTGGCTCCTGCACCTCCAG261520.1298723936194277No Hit
GTTCCACACAAAGGCCGAGGTCCGGAGTTCAGGTTCCACGATTTAGAAGA244430.12138539756958058No Hit
CCCACGGTGATCACGCGGCGCCAACGATCAGACATATAACGCCTGCGACG235860.11712948439537404No Hit
GGATAAGGAGTGGATGCCCGTCACCAAGTTGGGCCGCTTGGTCAAGGACA204090.10135231268655935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)