FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15157477
Sequences flagged as poor quality0
Sequence length50-120
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGACCAAGGAGTCTAACATGTGTGCGAGTTTTTGGGTGGCAAACCCAT3131602.0660430492488953No Hit
CATGTGTGCGAGTTTTTGGGTGGCAAACCCATGAGCGTAATGAAAGTGAA2086841.3767726647383334No Hit
CTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATG1672831.1036335400673871No Hit
AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAACA1571961.03708552551325No Hit
CAATAACGCAGGTGTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCAG1233520.8138029831745744No Hit
TCCAGACATAGGAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATG875320.5774839704523385No Hit
ACGGGGAAACTCACCAGGTCCAGACATAGGAAGGATTGACAGATTGATAG858030.566077058866723No Hit
TGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAAC799210.5272711283018935No Hit
GGTCTCAAATCAGGTAGGATCACCCGCTGAACTTAAGCATATCAATAAGC659540.4351251860715342No Hit
ATCACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAAC558020.3681483402547799No Hit
GACTTCAAGTGCTTCCCTTTCGACAATTTCACGTACTTTTAACTCTCTTT532130.35106766119453786No Hit
AAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAA496110.327303811841509No Hit
ACATGTGTGCGAGTTTTTGGGTGGCAAACCCATGAGCGTAATGAAAGTGA486820.3211748234881043No Hit
TAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAAC332960.2196671649246111No Hit
GACACGGGGAGGTAGTGACAATATGTAACAATATACGGCTTTATCGTCGT287370.18958959990505017No Hit
CACGGACCAAGGAGTCTAACATGTGTGCGAGTTTTTGGGTGGCAAACCCA265450.17512809024879272No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG238080.15707099539059172No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA223350.1473530192392837No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA216950.14313068065351509No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA212390.14012226441115497No Hit
ACAATAACGCAGGTGTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCA190600.12574652100742095No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA184440.12168252011861867No Hit
CGTATCCTGCATAAGGCTATAACACGTCCGGAAACGCTACATTCCCTATG179480.11841020771464802No Hit
CCTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCAT171030.11283540130062543No Hit
ATGCCTGAATAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCG162480.10719462084620021No Hit
TGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTT156260.10309103553315634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)