FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15157477
Sequences flagged as poor quality0
Sequence length50-120
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGACCAAGGAGTCTAACATGTGTGCGAGTTTTTGGGTGGCAAACCCAT2137351.4100961525457039No Hit
CATGTGTGCGAGTTTTTGGGTGGCAAACCCATGAGCGTAATGAAAGTGAA1732041.142696769389787No Hit
AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAACA1362310.8987709498091271No Hit
CTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATG1186400.7827160153368533No Hit
CAATAACGCAGGTGTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCAG924690.610055354199119No Hit
TCCAGACATAGGAAGGATTGACAGATTGATAGCTCTTTCTTGATTCTATG664040.43809401788965274No Hit
TGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAAC614860.40564798481963715No Hit
GGTCTCAAATCAGGTAGGATCACCCGCTGAACTTAAGCATATCAATAAGC576290.38020179743634114No Hit
ATCACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAAC543590.35862828622467974No Hit
ACGGGGAAACTCACCAGGTCCAGACATAGGAAGGATTGACAGATTGATAG538810.35547472709343386No Hit
GACTTCAAGTGCTTCCCTTTCGACAATTTCACGTACTTTTAACTCTCTTT488440.3222436029426269No Hit
AAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAA465040.30680567748841053No Hit
ACATGTGTGCGAGTTTTTGGGTGGCAAACCCATGAGCGTAATGAAAGTGA433800.2861953872666276No Hit
TAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAAC294730.19444528927868404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG247800.16348367211772777No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG245110.1617089704308969No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA209630.13830138089604227No Hit
GACACGGGGAGGTAGTGACAATATGTAACAATATACGGCTTTATCGTCGT195520.12899244379523056No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA192750.1271649628760776No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA180310.1189577922499899No Hit
ACAATAACGCAGGTGTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCA172680.1139239729672689No Hit
CGTATCCTGCATAAGGCTATAACACGTCCGGAAACGCTACATTCCCTATG164270.10837555616940735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)