FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004455731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24565044
Sequences flagged as poor quality0
Sequence length50-120
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA1686000.6863411276609153No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA879160.35789066773094325No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA686580.27949471615031507No Hit
CGGCTGCAGCTGCTGCTGCTGAGCCGCCCCCTTTCTCGGGCCCAGCCACA650780.2649211619568033No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC566520.23062038887453246No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC484030.19704015185154972No Hit
AAGGCTCGGTGGCTGCTGCGGTCCCAGACCCTTCACCGGCCCTTGGGACC354190.14418455753631054No Hit
TTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTG349560.14229976547161893No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC317100.12908586689280915No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGT279290.11369407683536002No Hit
TGGTGGTCGTACGCTGTGAAGGCATCAACATTTCTGGCAATTTCTACAGA275280.11206167593267896No Hit
ACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAATAAA255210.10389152976888623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)