FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004455732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13073543
Sequences flagged as poor quality0
Sequence length50-120
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTACACATTTAAAGTTTGAGAATAGGTTAAGGATGTTACATCCCCAAG700540.5358455622932513No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG453250.3466925530439606No Hit
TAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAATGAATTGAC395270.3023434427836433No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC295170.22577659323107746No Hit
CATGTGTGCGAGTTTTTGGGTGGCAAACCCATGAGCGTAATGAAAGTGAA288920.22099594578149168No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAG253290.19374243080089307No Hit
GCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATG246300.18839575469327632No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA233260.17842141185446056No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC218610.1672155742326315No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA197340.15094607483220118No Hit
CTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACA196170.15005113762963873No Hit
TGAGCCTAAATCAGATTAGCTAGTTGGTGGGGTAAAGGCCCACCAAGGCG190350.14559939872458447No Hit
ACGGACCAAGGAGTCTAACATGTGTGCGAGTTTTTGGGTGGCAAACCCAT180250.13787387244605384No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGT155460.1189119124020168No Hit
GGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGAA153220.11719852835608527No Hit
CATCGACCGGCCCTGATGTTCTCGGATGGGTCTGCGTAAGAGCATATATG152450.11660955259029629No Hit
AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCA139500.10670405107475456No Hit
AGAAAGTGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA138780.10615332048856227No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT138060.10560258990237001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)