FastQCFastQC Report
Tue 26 Sep 2023
EGAF00004455734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37590272
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA2248480.598154756634908No Hit
ATCTAAGTACCCGTAGGAAAGGACATCAACCGAGACTCCGCAAGTAGTGG1318970.35088067465965667No Hit
TTCTCTACCTGACCACCTGTGTCGGTTTGGGGTACGATTTGATGTTACCT1145320.3046852121740433No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA997530.26536918913489105No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG925860.24630308607503557No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA911370.24244836536431555No Hit
GACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT799820.21277313449607388No Hit
AGAAGGCACGCTGATATGTAGGTGAAGTCCCTTGCGGATGGAGCTGAAAT669830.17819237913468675No Hit
TCTCTACCTGACCACCTGTGTCGGTTTGGGGTACGATTTGATGTTACCTG664770.17684628618808612No Hit
GACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGC662860.17633817600468546No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA640910.1704988992896886No Hit
CATCTAAGTACCCGTAGGAAAGGACATCAACCGAGACTCCGCAAGTAGTG620400.1650427004092974No Hit
CCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGAT550510.14645012411721842No Hit
GGAGGCAGCAGTAAGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCC537250.1429226157235574No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC521870.1388311316289491No Hit
GACAGAGCCACAAGACAGGAGCCCAGCACCCATGAGTTGTGACAAATCAA518030.13780959073666718No Hit
GGATACAGCTTTGCTCCTCCAGAGCCAAAATACATAGGATGTGGCTGAGA441430.11743197814583518No Hit
GTCAGCGCCCACACCCCGGCCAGCTTTTAATGTGAACAACCCGTTGTGTT416540.11081058418518494No Hit
GGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAA408960.10879410502802427No Hit
CTTGTTGGTTTGAACAAGCAAAATGTCTGCATGGTATCTCGGCTCCGCAA405340.10783108991602934No Hit
TACTCTACCTGACCACCAGTGTCGGTTTCGGGTACGGTCTATAAGTGGGA403920.10745333260690426No Hit
ACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACG387770.1031570082812915No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC385650.10259303258034419No Hit
GCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGCA376820.10024402058064384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)