FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15251089
Sequences flagged as poor quality0
Sequence length50-120
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCAGAGACCCCTGAGGGCAGCAGATCATCATAATAGATGAGGAGT4535962.9741876137500736No Hit
GCCCCAGAATTCCCAGGAGTTTCCATTCGGTGATCAGCACTGAACACAGA1017580.6672179278476442No Hit
TCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTG881770.5781685491442612No Hit
AGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGATCAGCACTGAACACAG837260.5489837479802262No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG785370.5149599481060008No Hit
CCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGAT702600.4606884137913037No Hit
TGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC663270.4349000913967521No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA654800.42934638962502936No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAG601080.3941226754364885No Hit
CTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGT506320.3319894074449372No Hit
CTTCAGCTGCGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGC503140.3299043104397332No Hit
CTGCGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTC483460.3170003138792253No Hit
ACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTG414920.2720592608173751No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC399340.2618435968736396No Hit
AAGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCT388680.2548539320700312No Hit
GGACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCT353200.23159001957171713No Hit
GCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCA319760.20966371647296791No Hit
AGGCTGATTATTATTGTGCTGCATGGGATAACAGCCTGAACGGTTGGGTG270990.1776856721510182No Hit
CAGCTGCGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCTG205250.13458055355915896No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG191540.12559103156502466No Hit
CAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGATCAGCACTGAACACA182140.11942753727291211No Hit
CTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGC177240.11621465195042795No Hit
TGCGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTCC176040.11542782289186038No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG175330.11496228236554124No Hit
TAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAA172690.11323125843669263No Hit
GGAGGCAGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCC160460.10521215894812494No Hit
GACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT158380.10384832191327452No Hit
TGGTGTCGATATTGTCATGAACCATCACCTGCAGGAAACAAGTTTCACAA155290.10182223708746307No Hit
GCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGT153770.10082558694661083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)