FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15251089
Sequences flagged as poor quality0
Sequence length50-120
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCAGAGACCCCTGAGGGCAGCAGATCATCATAATAGATGAGGAGT4143972.7171633448601606No Hit
GCCCCAGAATTCCCAGGAGTTTCCATTCGGTGATCAGCACTGAACACAGA997440.654012313481352No Hit
AGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGATCAGCACTGAACACAG786720.5158451307968893No Hit
CCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGAT717630.47054344774986234No Hit
TCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTG691610.45348237099658917No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA686020.4498170589654287No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG656210.4302709137688463No Hit
TGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC603910.39597828063294366No Hit
CTTCAGCTGCGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGC526630.3453064892611931No Hit
CTGCGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTC448000.293749515198554No Hit
AAGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCT421370.2762884670071757No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAG413940.27141668375287825No Hit
CTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGT394980.2589847846275109No Hit
ACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTG383820.2516672743828326No Hit
GGACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCT364370.23891408672521683No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC357700.23454062854134544No Hit
AGGCTGATTATTATTGTGCTGCATGGGATAACAGCCTGAACGGTTGGGTG288930.18944876657660314No Hit
GCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCA255760.16769950001603162No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG225450.14782550937837946No Hit
CAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGATCAGCACTGAACACA187950.12323710129814337No Hit
CAGCTGCGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCTG186830.12250272751014699No Hit
TGCGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTCC161570.10593997582729994No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG155500.10195993217271239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)