FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004455738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213840
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCAGGTTCGGAATAACAGCGAGAAATTGCTGCTAATACCGGATGA312421.4112130957973474No Hit
CAAGCAAGCTTAAGCCGTTAGGTGTAGGCGCAGCGAAAGCGAGTCTGAAT234421.0588841108661873No Hit
CTCTAAGCCTAAATACTCGTATGCGACCGATAGTGAACTAGTACCGTGAG165810.7489701152748166No Hit
AGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACACAG155630.7029866657030318No Hit
CTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGT83460.37699201387634157No Hit
TACTCTACCTGACCACCTGTGTCGGTTTCGGGTACGGTCTATACGGTGGG80350.3629440248617786No Hit
TGCAAGTCGAGCGGGCGTAGCAATACGTCAGCGGCAGACGGGTGAGTAAC78370.35400028909044917No Hit
CCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTAC77300.3491670581433166No Hit
ACTCTACCTGACCACCTGTGTCGGTTTCGGGTACGGTCTATACGGTGGGG76850.3471343909225599No Hit
CTGCTTGTAGGCAATCCGTTTCAGGTACTGTTTCACTCCCCTCATCGGGG62360.28168250641419434No Hit
CACTTTCGCATACGGGGCTGTCACCCGCTATGGCCGATCTTTCCAAATCG58350.263569182958118No Hit
ACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTA55940.2526831207313988No Hit
AATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCC47430.21424312506775559No Hit
GCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACACAGA43900.1982979799804864No Hit
AAGAAGGAGGCCCTCATTGTGCGCAAGCATTTTGAGGGGGCACAGGCCAG43310.19563292740216093No Hit
AGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGC42400.19152242257796406No Hit
AGAAGGAGGCCCTCATTGTGCGCAAGCATTTTGAGGGGGCACAGGCCAGG39330.17765511509413506No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG37310.1685306977920717No Hit
GGTCCCCTTGCTCTAGAAGTGGCAGCATATCTTCTGCACCAAGAGGAGGG35900.16216167383370073No Hit
CCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACT34160.15430202724677483No Hit
ACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACG33900.1531275972970043No Hit
CAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACACA32060.1448162468832436No Hit
CGAGCCCGCTGCTACTCGGGGACACCAACAACACAACCAACACCACCTTC30180.13632421493874897No Hit
TCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTG30090.13591768149459763No Hit
CAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTC30070.13582734072923067No Hit
GTAGGTCATCATCGCACCCTTGAAAGTAGCCCATGCCTTCCAAAGCGATT29060.13126513207819898No Hit
CTTTAGCTGAGACAAGCAGGTCTGGTGGGCTGACCATTTCTGGCCATGAC28980.13090376901673112No Hit
GTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCTGGAGGAAAAGAC28440.12846456835182307No Hit
ATCGCCTGCACGCCCGAAGTTAAGCCCCGGAATTTCACAGACGACGCGAC26240.11852708416145702No Hit
GATATCAGGAGGAACACCGATGGCGAAGGCAGGTCTCTGGGCAGTAACTG26060.11771401727315434No Hit
CATGCCATCCCCATTCAGAGGTTGTGGAGCAGCTCGTGCGACCTCTCCTT25560.11545549813898022No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA24320.10985437068622847No Hit
AGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT24260.10958334839012757No Hit
GATATTCGCAAGAACACCAGTGGCGAAGGCGGCTCACTGGCCCGATACTG23570.1064665919849673No Hit
AAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAG22630.10222057601271999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)