FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004455739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2213840
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCAGGTTCGGAATAACAGCGAGAAATTGCTGCTAATACCGGATGA211270.9543146749539263No Hit
CAAGCAAGCTTAAGCCGTTAGGTGTAGGCGCAGCGAAAGCGAGTCTGAAT154980.7000505908286054No Hit
AGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACACAG140580.6350052397643913No Hit
CTCTAAGCCTAAATACTCGTATGCGACCGATAGTGAACTAGTACCGTGAG129490.5849112853684096No Hit
TACTCTACCTGACCACCTGTGTCGGTTTCGGGTACGGTCTATACGGTGGG80440.36335055830592994No Hit
ACTCTACCTGACCACCTGTGTCGGTTTCGGGTACGGTCTATACGGTGGGG79660.3598272684566184No Hit
CCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTAC73850.33358327611751526No Hit
CACTTTCGCATACGGGGCTGTCACCCGCTATGGCCGATCTTTCCAAATCG67130.3032287789542153No Hit
TGCAAGTCGAGCGGGCGTAGCAATACGTCAGCGGCAGACGGGTGAGTAAC61140.27617171972680954No Hit
CTGCTTGTAGGCAATCCGTTTCAGGTACTGTTTCACTCCCCTCATCGGGG56650.25589021790192606No Hit
ACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTA54690.24703682289596357No Hit
CTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGT54300.2452751779713078No Hit
AATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCC41650.18813464387670292No Hit
GCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACACAGA41130.18578578397716186No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG37250.1682596754959708No Hit
GGTCCCCTTGCTCTAGAAGTGGCAGCATATCTTCTGCACCAAGAGGAGGG35020.1581866801575543No Hit
CCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACT33750.15245004155675207No Hit
CAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACACA31700.14319011310663823No Hit
CGAGCCCGCTGCTACTCGGGGACACCAACAACACAACCAACACCACCTTC31660.14300943157590432No Hit
AAGAAGGAGGCCCTCATTGTGCGCAAGCATTTTGAGGGGGCACAGGCCAG30640.13840205254218915No Hit
ATCGCCTGCACGCCCGAAGTTAAGCCCCGGAATTTCACAGACGACGCGAC29790.13456257001409316No Hit
AGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGC29760.1344270588660427No Hit
AGAAGGAGGCCCTCATTGTGCGCAAGCATTTTGAGGGGGCACAGGCCAGG29250.13212336934918512No Hit
CAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTC28190.12733530878473603No Hit
GTAGGTCATCATCGCACCCTTGAAAGTAGCCCATGCCTTCCAAAGCGATT26270.11866259530950746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24650.11134499331478336No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA24510.11071260795721462No Hit
AGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT24340.10994471145159541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)