FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004455742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4191742
Sequences flagged as poor quality0
Sequence length50-120
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACT470821.1232084417409278No Hit
CTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATG292750.698396991036185No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA165610.3950863388061575No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA110940.2646632354758475No Hit
TAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAAC100600.23999568675743876No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC87300.2082666347308589No Hit
CTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTAT85840.20478359593696366No Hit
GACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCTTATGAGTC75560.180259185799126No Hit
CATGTGTGCGAGTCAACGGGCTAGTAAACCCGTAAGGCGCAAGGAAGCTG73120.17443821685590383No Hit
CATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCC70460.16809240645058784No Hit
GGAGGGTCACCTCCGCATAGCTAGTTAACAGGCTGAGGTCTCGTTCGTTA65840.15707073574661798No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA60540.14442682779617638No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC50620.12076124914176492No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG47570.1134850379627372No Hit
ACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAA45700.10902388553493988No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA42900.10234408510829149No Hit
ACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGCTAGTAAACCCG42240.10076956072201008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)