FastQCFastQC Report
Sat 1 Apr 2023
EGAF00004455744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17458668
Sequences flagged as poor quality0
Sequence length50-120
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAAC1589230.9102813570886393No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG890580.5101076439508444No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA606850.34759238219089794No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA547740.31373527464981865No Hit
GATATTCGCAAGAACACCAGTGGCGAAGGCGGCTCACTGGCCCGATACTG471050.2698086703979937No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA467770.26792994746220045No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA393760.22553839731645048No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG354300.20293644394864485No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA323520.18530623298409707No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCC230060.1317740849416462No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC229610.13151633331935747No Hit
TCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC193600.11089047572243196No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC188290.10784900657942519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)