FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004455751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26795923
Sequences flagged as poor quality0
Sequence length50-120
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA2339960.8732522481125207No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA1970590.7354066512282484No Hit
TCCCCCAGGACCCTCGGGCGTGCCCCCCGGGATGCCAGGCCAGCCTCCTG842100.31426422594213305No Hit
GCCTGCAGCCTGTACTCGCGCTCCTGCAGGATCTCCACAGGGTCGAGGCC390400.1456938057330587No Hit
CACCAACCGGGGCCCCAGGCTCCGCCCCTCACATCCTGTGCCCGGTTTCC382940.1429098001214588No Hit
TGTGATTTCCAATTCGGAGCATTTTGTACCTCTCTGCACTCTTCATATGT366310.13670363211597525No Hit
ACATTTTGGGAGGCAACCGCCCCAGTTAAACTACCCACCAGGCACTGTCC329000.12277987214696802No Hit
AGCCTGCAGCCTGTACTCGCGCTCCTGCAGGATCTCCACAGGGTCGAGGC308600.1151667736916545No Hit
TCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC281580.1050831501493716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)