FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15305942
Sequences flagged as poor quality0
Sequence length50-120
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCCGGGCCTTTCCTTCTGGGGAGCCGCATGCCCTTCACTGGGCGTGT3395682.218537088406581No Hit
AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCA623050.4070641323480776No Hit
CTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATG583310.38110035958583927No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA518820.3389663961878335No Hit
CATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAAAGTGAA506410.3308584339336971No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC432220.28238706248854206No Hit
CTGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCT424320.2772256683058122No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA364560.2381820080070864No Hit
GATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAAC358970.23452983161702823No Hit
ACGGACCAAGGAGTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCT316530.2068020380581607No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA313820.20503148385117362No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA280190.18305962481760352No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC276730.18079906483377503No Hit
ACATAGCATTGCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTG254240.16610542493888975No Hit
CATAGCATTGCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCTGC229290.14980456609596457No Hit
GACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGC195620.1278065734209629No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC191560.12515400881566127No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG183160.11966594411503716No Hit
TCAAGACGTGCAACAAGGGTGACCTTCAGTGCTGCAACCAGGTCCAGACG176810.11551722853777964No Hit
CTCGTAAGGTGCCGATGGAGTCAAAATAATAACATCCACCGATCCCTAGT175790.11485082068127528No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT170510.11140118001231156No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG162660.10627245288137117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)