FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004455756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493534
Sequences flagged as poor quality0
Sequence length50-120
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG242290.6935384055228888No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC208560.5969886080971303No Hit
CCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGAT161040.46096588726487275No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA139090.3981355269477841No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA111140.3181305806670266No Hit
TGCAAGTCGAACGAGATCCTTCGGGGTCTAGTGGCGCACGGGTGCGTAAC103220.29546012719498366No Hit
GCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCA90400.25876376185261113No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG79510.22759188832855212No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG57610.16490464956116072No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC51430.14721482601858174No Hit
TGATGAAGGTTGGTGAGGCCAACCCAAAGCTGCAGAAAGTACTTGATGCC45720.1308703450431569No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA43840.12548897477454063No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC41460.11867638900895196No Hit
GCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGT40980.11730242213185844No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC40140.11489798009694481No Hit
ACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAA36950.10576682522626085No Hit
CTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCG36770.10525158764735079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)