FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004455759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12797911
Sequences flagged as poor quality0
Sequence length50-120
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA407520.31842696827630695No Hit
CATGGAAACCCAGGCTGGGGAGCCGCCTCTTGTCTCACAAAAGTCCACGT329380.25737012860927067No Hit
CAGCCTGGATGACAGAACAAGACCTTGTCTCAATAAAAAATAATTTGCCA314730.24592294789360541No Hit
ACAGAATGCCACCCCACCAACCCAGTTTCTAAAGCGAGAAAACTAGAAAC235670.18414724090517584No Hit
TAACTGGTACCCGGAACTGCTGGATAACGTTGAACCCAGCTTTGTCTGGG231960.181248330293905No Hit
GGACAGGAACCCTTGGTCTTCCGGCGAACGGGTTTTTCACCCGTTTTATC214890.16791021597196604No Hit
AACGGGTTAATATTCCCGTACTTGGATAAACTGCGATGTGGGGACGGAGC212620.16613648899418038No Hit
CTACAAAACAATTTGGAAAAGAAGAAAAGGTTAAAAAACCGTAATAGCCA203180.1587602851746664No Hit
TTGGGAGGATAGTTAATTAACAAATGGACGTGATCTGGTTCGCCATCCAT188370.14718808405528058No Hit
AATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCATAATGATGGCC188230.14707869120202507No Hit
AGAAGGTGCGCCGGCGTAGATTGTAAGGGCTTGCCCCTGAAGGTTGAACC183010.14299990053064127No Hit
TCCACAAAAAATTAAAACCAAATAAATTAGCCAGCCGAACATGGTGGTTC181500.14182002047052836No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA177240.13849135222146802No Hit
CGGGGCACCACTCATCATCTTTCCAATTTGCTATAGCTATGCTCCTCTTT162030.12660660009278077No Hit
TTAATGGACAATACTGCATACCTAGAAAAGGAAAAGACTTCACCATCCCT159290.12446562567906591No Hit
ACGTTATGGCGACCAAACGCCTTTTCGGGGCTACCCGGACGTGGGCCGGC155790.1217308043476783No Hit
CGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGA155540.1215354599668649No Hit
TGCAGGAGGCAGTGCCTGGCTCCATCCGCACGGCCGAGCATTTCCTGGGC142300.11119002155898725No Hit
CACTGCAGCCTCAAACTCCCGGGGCCCAGGTGATCCACCTCGGCCTCCTG140360.10967414916387526No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC140000.10939285325550396No Hit
GGTTGGCAATGGTCGGGGTTCTGTCGTCAAAGGGCTCGATGATGATGGTG132140.10325122592273067No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC129520.10120401681180623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)