FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004455760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12276459
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA964420.7855848335419847No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA958150.780477497623704No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA953560.776738634487355No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA766190.6241131909453695No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC498150.4057766168567011No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC451070.36742679627733044No Hit
TGCCGAAAAGAGAGGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAG430590.3507444614118778No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG417040.33970707677189327No Hit
TGTAATCCGTCTCCACAGACAAGGCCAGGACTCGTTTGTACCCGTTGATG349150.2844061141734763No Hit
GGAAAGGAATGGCTGGGGATAGGCGAAGATAGGACCATGGCTAGAGGCAC328570.26764232259481335No Hit
GAGAACTCGCCCAGGTAGTGGCCGATCATCTCGGGCTTGATCTCCACCTG314470.25615692603217266No Hit
CTCGGGAATATGTGGCCTGGACTGGATGGGGTGGTAGGGGAGGAGCGAGC237430.19340267417502066No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT229310.18678838906235096No Hit
GCTACCTGGCCGAGAAGTACGAGTGGGACGTGGCTGAGGCCCGCAAGATC216450.1763130557435169No Hit
TAACATCTCAGAAATCAGATTTATTTTACAAGGGTGTATCACTGTTAATG215700.1757021303944403No Hit
TCAAAGGCAATTCCGAGGTTGAGCCCCGGGATTTCACCCCTGACTTAAAT211430.17222392874036396No Hit
CTGATCATGCTGCTCCTGTCACCCTCCACGATTCCACAGACCAGGACATT203670.16590288779525106No Hit
GCAGCTGCAGGATCAGGAACAGTGAGAACAGCACGCCGATCGCCGGAACC187980.15312232949256785No Hit
GGACCGGCGCGGCGTGCCGCAGCGGGTGACCTGGATCGCCGGCGTCGGCT187020.15234034504574975No Hit
GGCCATGCCTTTCAAGTGCATGCAGCTCACTGATTTCATCCTCAAGTTTC185670.15124067941741182No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG172080.14017071209214318No Hit
TGGTGGTCGTACGCTGTGAAGGCATCAACATTTCTGGCAATTTCTACAGA170400.13880223931021152No Hit
AAAAGAAGAAGATCCGCACGTCACCCACCTTCCGGCGGCCGAAGACACTG163630.13328761982587975No Hit
CCCAGGAGGACCAGGGTGCAGGGGAGGTGGAGAGAACCTCGAGGGCCGTG163060.13282331656058152No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC153220.12480797598069607No Hit
CAAAAGGAATCGTGCTGGAAAAAGTAGGAGTTGAAGCCAAACAGCCAAAT150200.12234798324174748No Hit
TGCCCCATGTGAAGTCACTGTGCCAGCCCAGAACACTGGTCTCGGGCCCG145440.11847064369294111No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG144700.11786786401518548No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG136000.11078112996589652No Hit
TCTTGGAGGAAACATTGTGAGCGATCTCGGCACAGTAAGATTTGTTGCAC133030.10836186558355304No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCC124540.10144619063200554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)