FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004455761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12276459
Sequences flagged as poor quality0
Sequence length50-120
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA907390.7391300699981973No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA847970.6907284910086858No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA660870.5383229805923679No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA441600.3597128455363228No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC426850.3476979803378156No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC374780.30528346976925513No Hit
TGTAATCCGTCTCCACAGACAAGGCCAGGACTCGTTTGTACCCGTTGATG361890.29478369943645805No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG350940.2858641893399392No Hit
GAGAACTCGCCCAGGTAGTGGCCGATCATCTCGGGCTTGATCTCCACCTG313870.25566818575291134No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT277970.22642522571044307No Hit
GGGCATCCTCCAGTCGCTTCCCTCGAAGTTCCAGCTCGTGGCCCAGGCGT214480.17470835849327562No Hit
TCAAAGGCAATTCCGAGGTTGAGCCCCGGGATTTCACCCCTGACTTAAAT209270.17046446373502327No Hit
GCAGCTGCAGGATCAGGAACAGTGAGAACAGCACGCCGATCGCCGGAACC203280.1655852066137312No Hit
GGACCGGCGCGGCGTGCCGCAGCGGGTGACCTGGATCGCCGGCGTCGGCT187310.15257656951405937No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA176470.14374666180207177No Hit
GCAGGAGCTCCACGTCCTGCCAGCGTTTCCGGGCGACGCCTTCGCCGACC170390.1387940936388905No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG169310.13791436113622016No Hit
CCCAGGAGGACCAGGGTGCAGGGGAGGTGGAGAGAACCTCGAGGGCCGTG168450.13721383340261226No Hit
TGCCCCATGTGAAGTCACTGTGCCAGCCCAGAACACTGGTCTCGGGCCCG159640.13003749696879208No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG154860.12614386607734362No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC144700.11786786401518548No Hit
ACATCACTGTCGTGGGACTGAGGTCATTGCGGGTGCCCAGCAGCACGATG129020.1050954513838233No Hit
AGATGGCAGAAGTAGAGCAGAAGAAGAAGCGGACCTTCCGCAAGTTCACC127090.1035233368188661No Hit
CCCAGCAGCCAGTGGGTTCCCGCGCGTGCCGAGACTCTGAGGCCTTGCAC124230.10119367482105386No Hit
TGCCGAAAAGAGAGGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAG123520.1006153321572613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)