FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004455770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4325638
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA386420.893324869071337No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA367190.8488689992089028No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC209580.48450656296250405No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC178460.4125634183905357No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC129820.3001175780312638No Hit
CTGATCTTCGTGGTGGACAGCAATGACAGAGAGCGTGTGAACGAGGCCCG123440.2853683086749284No Hit
GCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCA113150.26157991029300187No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG110940.2564708373654938No Hit
GAGAACTCGCCCAGGTAGTGGCCGATCATCTCGGGCTTGATCTCCACCTG104540.2416753320550633No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC96340.22271859087607423No Hit
TTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTG92380.21356387196524534No Hit
TCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA86740.20052533291042848No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCC86560.20010920932357262No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG77290.1786788446004959No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC74310.17178968744032672No Hit
CTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTG74030.1711423840829954No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT72820.16834510886024212No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA69010.15953715960512646No Hit
CTACCACGGGTAATGAGAGGTCACTCACTTGAACTTTGCCATTCCAGGCA68930.15935221578874606No Hit
TGCCGAAAAGAGAGGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAG67180.15530656980542523No Hit
CGGTAATGCTGGTGGTCTGCATCACCACCGGATCAACTTCGACAAATACC57660.13329825565615985No Hit
GGCAAATATCCTGTGATCCGGAGATGTCTGAATGGGGAAACCCACTTACC56300.13015421077769337No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG55370.12800423891227145No Hit
AATGTCTCTAGACAAAAGAGAAGAGAGTTGGAGGTAGATTGTTTGGTGAC55070.127310699600845No Hit
TAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTAC52690.12180862106352867No Hit
CAGCAACTGCTTTCTTAATTAAATGGTAGAGATCTTCAGGAAGATCAGGA52100.12044466041772335No Hit
GGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTAGGCGGGT51440.11891887393258521No Hit
TGGTGGTCGTACGCTGTGAAGGCATCAACATTTCTGGCAATTTCTACAGA50400.11651460431964025No Hit
TAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTT49850.11524311558202512No Hit
CGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAA48900.11304690776250809No Hit
TTATTACACGTTTTAAGAAGATAAGCTAATTCTTGAAACTCACCAAAGAG48540.11221466058879638No Hit
GGATCGGTCTCAAAGCCATGATCAGTTTGGGGAGAACCAAAAGCATCCCC48210.11145176734622732No Hit
CTGGGCTCGGGCAGCTGCAGGGCCAGGTAGTCAGAAGGGAATGGGGGGTA47520.10985662692994652No Hit
CGCTCAGACACACACAGTGTATCGCGTATGTCGAGTATCCATCGTTTAGG43570.10072502599616519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)