FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004455771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4325638
Sequences flagged as poor quality0
Sequence length50-120
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA334600.773527512010945No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA307690.7113170357759941No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC166840.3857003290612853No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC143190.33102631334383503No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC126990.2935751905268078No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG122790.2838656401668378No Hit
GAGAACTCGCCCAGGTAGTGGCCGATCATCTCGGGCTTGATCTCCACCTG117470.27156687637754245No Hit
CTGATCTTCGTGGTGGACAGCAATGACAGAGAGCGTGTGAACGAGGCCCG100350.23198889967214084No Hit
GCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCA92870.2146966528405752No Hit
TCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA87630.20258283286766024No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC77590.17937238391192237No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA75950.17558103567612454No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT74400.17199774923375466No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCC73840.17070314251909197No Hit
TTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTG72460.1675128616865304No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG66270.1532028338940984No Hit
CTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTG61650.14252232849813137No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC60990.14099654201299325No Hit
CTACCACGGGTAATGAGAGGTCACTCACTTGAACTTTGCCATTCCAGGCA56450.1305009804334066No Hit
AATGTCTCTAGACAAAAGAGAAGAGAGTTGGAGGTAGATTGTTTGGTGAC54760.12659404231237104No Hit
TGGTGGTCGTACGCTGTGAAGGCATCAACATTTCTGGCAATTTCTACAGA48960.11318561562479337No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG47500.10981039097585142No Hit
TAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTAC45480.10514055961224679No Hit
CGCTCAGACACACACAGTGTATCGCGTATGTCGAGTATCCATCGTTTAGG45150.10437766636967771No Hit
TGCCGAAAAGAGAGGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAG44810.10359165515006111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)