FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004455776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4973701
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACACGGCCGTGTCCTCGGCTCTCAGGTTACTCATTTGCAGATACAG1108872.229466548149959No Hit
AGCCTCCACCAAGGGCCCATCGGTCTTCTCCCTGGCGCCCTGCTCCAGGA1001492.0135709806439914No Hit
AGTCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGA623221.253030690827615No Hit
AGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTG391920.7879846416179823No Hit
ACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGC330340.6641734193511029No Hit
CACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT208370.4189435593333817No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT203480.40911184649016896No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG192710.3874579513324183No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA139970.28142021404181716No Hit
TAGTCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAG139250.2799725998808533No Hit
GTCTCAGAGAGGAGCCTTAGTCCTGGACTCCAAGGCCTTTCCACTTGGTG128270.2578964839261548No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT122210.24571239807137582No Hit
CAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCT119510.24028384496776142No Hit
ACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTC98410.19786070775062672No Hit
TTTGGGGGGAAGAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGGGCA97790.196614151112019No Hit
GACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTG85830.17256767143823082No Hit
GCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATG84470.169833289134188No Hit
GTCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAG77200.15521640725890035No Hit
GAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGC76810.15443228292171163No Hit
AGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTG75280.15135610282966347No Hit
TTTTGGGGGGAAGAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGGGC72890.14655082804535294No Hit
GCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTA72730.1462291360095832No Hit
TTGGGAGGATAGTTAATTAACAAATGGACGTGATCTGGTTCGCCATCCAT72350.14546511742463006No Hit
GTTTTGGGGGGAAGAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGGG71090.14293179264294334No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA63870.12841543952883377No Hit
AGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTG62610.12588211474714703No Hit
GGTTTTGGGGGGAAGAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGG61460.12356995324005202No Hit
GGAGGCAGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAACCAGCC60420.12147895500754871No Hit
AGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGA60100.12083557093600923No Hit
AGACACTGGCGTGCCTGCTTCAAAGCCTCCCACCTATCCTACACAGGTAA58690.11800065987078838No Hit
AATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCT56970.11454247048626365No Hit
GAGAAGGTACGCTGCCGATGGTGAGTAGTCACGCACTACAAAGCTATTGG54750.11007899348995849No Hit
TCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCC54610.10979751295865997No Hit
GAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGAC54110.1087922253468795No Hit
AGGTGTTGCATGTGCGGGGTTGTGGGGGCCTTGTGGTTCTGCTGCCGCAG52330.10521340144894113No Hit
GGTAACCTTGGCTCCCGTTCCCAGTAAGTTCCTCATTTTCATCTGAGACC52250.10505255543105628No Hit
ATTTTGGAACCTTCCCTGTATACTGTCAAAGCCATCCTGATTCTGGACAA50980.10249912489713395No Hit
GCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGG50970.10247901914489835No Hit
CTGAGGACAATGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAA50760.10205679834795055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)