FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004455777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4973701
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACACGGCCGTGTCCTCGGCTCTCAGGTTACTCATTTGCAGATACAG1064062.139372672382196No Hit
AGCCTCCACCAAGGGCCCATCGGTCTTCTCCCTGGCGCCCTGCTCCAGGA920401.850533435765439No Hit
AGTCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGA528071.0617244583057968No Hit
AGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTG387480.779057687625372No Hit
ACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGC321080.6455554927809292No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT207530.41725467614559053No Hit
CACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT204290.4107404124212533No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG181750.36542204688219093No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA126880.25510178436540515No Hit
TAGTCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAG126150.2536340644522057No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT119700.240665854260238No Hit
ACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTC102670.2064257582029961No Hit
TTTGGGGGGAAGAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGGGCA88340.17761421524936863No Hit
GACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTG86690.17429676613049316No Hit
GTCTCAGAGAGGAGCCTTAGTCCTGGACTCCAAGGCCTTTCCACTTGGTG85250.17140153780856549No Hit
CAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCT80280.1614089789474679No Hit
TTGGGAGGATAGTTAATTAACAAATGGACGTGATCTGGTTCGCCATCCAT69900.1405392081269059No Hit
TTTTGGGGGGAAGAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGGGC67400.1355127700680037No Hit
GCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATG66740.1341857904204535No Hit
GTTTTGGGGGGAAGAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGGG66710.13412547316374668No Hit
GTCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAG65800.13229584971030628No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA62280.12521862492337196No Hit
GCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTA60440.12151916651201991No Hit
GAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGC59020.11866414969456346No Hit
GGTTTTGGGGGGAAGAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGG55730.11204935720904816No Hit
AGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTG52840.1062387948129572No Hit
AGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGA51990.10452980587293045No Hit
AGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTG51690.10392663330586217No Hit
AGACACTGGCGTGCCTGCTTCAAAGCCTCCCACCTATCCTACACAGGTAA50310.10115203949734816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)