FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004455780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26570018
Sequences flagged as poor quality0
Sequence length50-120
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAAC13072454.920000430560491No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG5620252.115260140207658No Hit
TACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGATCTGATGTGGAGG3406431.28205784429653No Hit
TCTCCAACATAGACTCAGTGAAATTGAATTCCCCGTGAAGATGCGGGGTT2632460.9907633483725906No Hit
AGCTTAGGGTGTGATCCGCAAGGGTCACGCGGTAGCGGAGCGTTCTGTAA2440780.918621884260673No Hit
CTTGACATCCCGGTCGCGGACTCCAGAGACGGAGTTCTTCAGTTCGGCTG2147210.808132685495358No Hit
ACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGATCTGATGTGGAGGC2098210.7896908462764309No Hit
GATATTCGCAAGAACACCAGTGGCGAAGGCGGCTCACTGGCCCGATACTG1775650.6682908532466934No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA1474230.5548471965656929No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA1325300.4987952962621252No Hit
GTAATGACTTCCCCGCTGTCTCCAACATAGACTCAGTGAAATTGAATTCC1234130.46448218439294997No Hit
ATAGACTCAGTGAAATTGAATTCCCCGTGAAGATGCGGGGTTCCTGCGGT1161560.4371694441456532No Hit
AAGCTTAGGGTGTGATCCGCAAGGGTCACGCGGTAGCGGAGCGTTCTGTA1070090.4027434230567702No Hit
TGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGG1056380.39758347171612757No Hit
ATGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAA862100.3244634610334099No Hit
CGCAGAACCTTACCAGCCCTTGACATCCCGGTCGCGGACTCCAGAGACGG849310.31964976463320427No Hit
AACTTGAGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAA683220.2571394569623551No Hit
GCGAGCGAACGCGGACCAGGCCAGTGATACATCAAAGACAATCGGAACCA623240.2345651403021255No Hit
CCTTGACATCCCGGTCGCGGACTCCAGAGACGGAGTTCTTCAGTTCGGCT599440.2256076755386466No Hit
CTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCG552230.20783952799730884No Hit
TCGAAGGCCATTCTGTGGTTGAGCCACAGGCTTTCACCCCCGACTTAAAA480960.18101606103541218No Hit
TCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC462320.174000634850906No Hit
GAGACTCCGTTAGTAGTGGCGAGCGAACGCGGACCAGGCCAGTGATACAT371420.1397891412794677No Hit
GAACGCGGACCAGGCCAGTGATACATCAAAGACAATCGGAACCAGTCAGG341320.12846058290212675No Hit
CCAACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATT323750.12184786626791146No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA299750.11281512869129406No Hit
GGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGG270140.10167098870614238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)