FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004455781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26570018
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAAC10422213.92254532909989No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG5843102.1991328722472074No Hit
TACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGATCTGATGTGGAGG3550871.336419869945139No Hit
ACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGATCTGATGTGGAGGC2195170.826183106085965No Hit
AGCTTAGGGTGTGATCCGCAAGGGTCACGCGGTAGCGGAGCGTTCTGTAA2087780.7857653690712593No Hit
CTTGACATCCCGGTCGCGGACTCCAGAGACGGAGTTCTTCAGTTCGGCTG1858100.699322070463031No Hit
TCTCCAACATAGACTCAGTGAAATTGAATTCCCCGTGAAGATGCGGGGTT1795170.6756374798090088No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA1586330.59703760832981No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA1356870.510677109816034No Hit
GATATTCGCAAGAACACCAGTGGCGAAGGCGGCTCACTGGCCCGATACTG1216510.4578506495554501No Hit
AAGCTTAGGGTGTGATCCGCAAGGGTCACGCGGTAGCGGAGCGTTCTGTA930860.3503422541904187No Hit
TGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGG888280.3343166722732367No Hit
CGCAGAACCTTACCAGCCCTTGACATCCCGGTCGCGGACTCCAGAGACGG851100.3203234563107936No Hit
GTAATGACTTCCCCGCTGTCTCCAACATAGACTCAGTGAAATTGAATTCC815140.30678940450849523No Hit
ATAGACTCAGTGAAATTGAATTCCCCGTGAAGATGCGGGGTTCCTGCGGT766730.28856962008832665No Hit
ATGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAA708580.2666840496683141No Hit
AACTTGAGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAA581620.2189008678880082No Hit
CCTTGACATCCCGGTCGCGGACTCCAGAGACGGAGTTCTTCAGTTCGGCT558900.21034987631547708No Hit
TCGAAGGCCATTCTGTGGTTGAGCCACAGGCTTTCACCCCCGACTTAAAA554920.20885194733402138No Hit
CTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCG495940.18665399473948419No Hit
GCGAGCGAACGCGGACCAGGCCAGTGATACATCAAAGACAATCGGAACCA453930.17084294033974684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333320.1254496703765876No Hit
TCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC323950.12192313908104993No Hit
AATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCGC295540.11123063597472911No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG289610.10899879706517324No Hit
AGAGCTTTACAACCCTAGGGCCTTCATCACTCACGCGGCATGGCTGGATC288290.10850199649845929No Hit
AAGAGCTTTACAACCCTAGGGCCTTCATCACTCACGCGGCATGGCTGGAT275250.10359420908183051No Hit
GAGACTCCGTTAGTAGTGGCGAGCGAACGCGGACCAGGCCAGTGATACAT273240.1028377173097888No Hit
CCAACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATT267770.10077900587045142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)