FastQCFastQC Report
Thu 9 May 2024
EGAF00004455787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27481851
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50-120
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTTTGGGAGGCAACCGCCCCAGTTAAACTACCCATCAGGCACTGTCC1184570.43103719614810515No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA784870.2855957555406294No Hit
TTCGGCGAAGCGGAGAACTTAAGCCCCAGTAAACGGCGGTGGTAACGATA545500.198494635605149No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA496760.18075929456134524No Hit
AATAAAGAAATTCTGGTGTGCCTCTGAGTCACACCAGCTGGGAACATGCT418350.15222773749846763No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA374050.13610800815418148No Hit
TAAATGCAATTGTATGCAACTGAAATCAAGAAGGAGTGTATCAGTTCTAA312850.11383876580947913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)