FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004455788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022158
Sequences flagged as poor quality0
Sequence length50-120
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA360421.1925915190403678No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA330171.0924974802773384No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC157430.5209191577673967No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC120770.39961510946813505No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC120360.3982584629923386No Hit
ACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAATAAA93660.30991099737340005No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG80710.2670608221012932No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA77520.2565054507408282No Hit
TGCCGAAAAGAGAGGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAG74990.24813394931701124No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG60000.19853363060435622No Hit
TTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTG58600.1939011792235879No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC57000.1886069490741384No Hit
GTTCATTTTTCTTTGCATAATCCAGGGAATCATAAATCATGCCAAAGCCA54110.17904424586669523No Hit
CGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCA52450.1735514820866414No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCC45510.1505877588134042No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT41550.1374845391935167No Hit
TTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCA40640.13447344579601728No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG39910.13205795329033096No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA37570.12431514169676104No Hit
CTAGGCAGAATAGTAATGAGGATGTAAGTCCGTGGGCGATTATGAGAATG36600.1211055146686573No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG32890.10882951850962126No Hit
TAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTT32230.10664564857297334No Hit
ATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTC31860.10542135785091315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)