FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004455789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022158
Sequences flagged as poor quality0
Sequence length50-120
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA357601.183260438401963No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA301680.9982270946787031No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC152750.5054335345802569No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC117300.3881332478315164No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC110980.3672210387411909No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG99570.3294665599879292No Hit
ACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAATAAA97510.32265023867051296No Hit
CGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCA53580.17729053212969012No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC52850.17487503962400378No Hit
TGCCGAAAAGAGAGGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAG52190.17269116968735587No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG50460.1669667833382636No Hit
TTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTG45370.15012451367532736No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA44760.14810608843084974No Hit
GTTCATTTTTCTTTGCATAATCCAGGGAATCATAAATCATGCCAAAGCCA43620.14433394944936698No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA42220.13970149806859866No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT40290.1333153329508252No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCC38210.12643283375654085No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG35060.11600981814981215No Hit
TTAGTCCGCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCA33490.11081485481566483No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG32620.10793611717190166No Hit
AACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTCCCCACCCC30690.10154995205412821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)