FastQCFastQC Report
Wed 8 May 2024
EGAF00004455794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5631388
Total Bases674.6 Mbp
Sequences flagged as poor quality0
Sequence length50-120
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA454010.8062133172141575No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA232650.4131308302677777No Hit
TGGTGGTCGTACGCTGTGAAGGCATCAACATTTCTGGCAATTTCTACAGA147300.26156961658475675No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG129200.2294283398693182No Hit
TGCCGAAAAGAGAGGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAG128160.22758154827903884No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG128110.22749276022181386No Hit
CCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGAT121790.2162699497885779No Hit
ACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAA114410.20316483254217257No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT111500.1979973676116794No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG111460.1979263371658994No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCC102680.182335154317195No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC101360.17999114960645582No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC95810.17013567525448434No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG95490.16956743168824454No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC95370.1693543403509046No Hit
GCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCA80180.14238052856595923No Hit
TTCAAAGTTTCCCAGGATCCCGATGTTGTCATACACTCCGAACATGGCCC79880.14184780022260943No Hit
GTTCATTTTTCTTTGCATAATCCAGGGAATCATAAATCATGCCAAAGCCA78150.13877573344262553No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC75210.13355499567779738No Hit
TGTAATCCGTCTCCACAGACAAGGCCAGGACTCGTTTGTACCCGTTGATG70160.12458740189807557No Hit
ACATCACTGTCGTGGGACTGAGGTCATTGCGGGTGCCCAGCAGCACGATG68070.12087606110607188No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA63400.11258325656125984No Hit
CGGTAATGCTGGTGGTCTGCATCACCACCGGATCAACTTCGACAAATACC60140.10679427523019192No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC57220.10160905268825376No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)