FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5631388
Sequences flagged as poor quality0
Sequence length50-120
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA453970.8061422867683776No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA232630.4130953150448877No Hit
TGGTGGTCGTACGCTGTGAAGGCATCAACATTTCTGGCAATTTCTACAGA147290.26155185897331173No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG129170.22937506703498317No Hit
TGCCGAAAAGAGAGGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAG128160.22758154827903884No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG128100.2274750026103689No Hit
CCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGAT121770.21623443456568792No Hit
ACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAA114380.2031115597078376No Hit
ACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT111490.1979796100002344No Hit
GGAATATCCTTCTCTCCTTTTTTTACAATAACCAAGTTGAGAACGCTCAG111460.1979263371658994No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCC102680.182335154317195No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC101360.17999114960645582No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC95790.17010016003159434No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG95470.16953191646535454No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC95340.16930106751656965No Hit
GCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCA80170.14236277095451422No Hit
TTCAAAGTTTCCCAGGATCCCGATGTTGTCATACACTCCGAACATGGCCC79880.14184780022260943No Hit
GTTCATTTTTCTTTGCATAATCCAGGGAATCATAAATCATGCCAAAGCCA78150.13877573344262553No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC75210.13355499567779738No Hit
TGTAATCCGTCTCCACAGACAAGGCCAGGACTCGTTTGTACCCGTTGATG70150.12456964428663059No Hit
ACATCACTGTCGTGGGACTGAGGTCATTGCGGGTGCCCAGCAGCACGATG68060.1208583034946269No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA63400.11258325656125984No Hit
CGGTAATGCTGGTGGTCTGCATCACCACCGGATCAACTTCGACAAATACC60140.10679427523019192No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC57220.10160905268825376No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)