FastQCFastQC Report
Mon 18 Sep 2023
EGAF00004455796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46681761
Sequences flagged as poor quality0
Sequence length50-120
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCAACTTTGCTTTTAATGTGGCCAACCCACTCCAGCCCCTCCCCTGGA10249862.1956883760233468No Hit
AAAAACACGTTGTATTTGCAAGTGAACAGCCTGAAACCCGAGGATACAGC5303011.135991849150678No Hit
AGCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAG4433040.9496299850384822No Hit
CTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAGT4361760.9343606381944332No Hit
GGATAGGAACCCTTGGTCTTCCGGCGGGGAAGTTTTTCACTCCCCTTATC3433250.7354585445052084No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGT2354430.5043575798265194No Hit
GGCATGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTA2055620.4403475695786198No Hit
TGCAAGTCGAGCGGGCGTAGCAATACGTCAGCGGCAGACGGGTGAGTAAC1872790.40118238041619725No Hit
GCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGAT1747340.37430892977666375No Hit
CGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGA1688060.3616101800444075No Hit
ATAACTAGAAGTACAGGAATATTAACCTGTTTTCCATCGACTACGCTTTT1666770.3570495123352352No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG1665400.35675603583163884No Hit
CTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACA1325830.28401456406068315No Hit
TCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAGTG1257780.2694371362725584No Hit
CAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAA1178770.25251189645566285No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA1044140.22367193902560786No Hit
ACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGC973960.2086382302501399No Hit
GAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAG970450.2078863305949405No Hit
CAGCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACA944900.20241310091108172No Hit
TAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAATGAATTGAC943530.2021196244074854No Hit
GGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGAGGACT914230.19584308312619142No Hit
AGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTG904690.19379945842231616No Hit
TAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAG890460.19075115868058193No Hit
ACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACG789510.1691260104776253No Hit
AACGGGTTAATATTCCCGTACCGACAATACTGCGATGGGGGGACGGAGAA709890.1520700986408803No Hit
TACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGGTCTAATGTGGAGG657130.1407680400060315No Hit
TCAATGGCAGTTTCATAGTTAAGCTATGAGATTTCACCACTGACTGATTA645890.13836024737798558No Hit
CAGACCCTGTCAGGACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCT635160.1360617051271909No Hit
GCTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAG612560.13122041389998118No Hit
AGCTTAGGGTGCATCGCAAGATGCGCGGTAGCGGAGCGTTCTGTAAGCCT603820.12934816233689214No Hit
AACGGGTTAATATTCCCGTACCGGCAATACTGCGATGGGGGGACGGAGAA588700.12610920997603325No Hit
GAGTCAGACCCTGTCAGGACACAGCATAGACATGAGGGTCCCCGCTCAGC572160.12256607029027888No Hit
CTTGACATGTTTGGGACCGGTCGCAGAGACGTGACCTTCTCTTCGGAGCC563850.12078593179036241No Hit
CGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGA561130.12020326311168938No Hit
GATATTCGCAAGAACACCAGTGGCGAAGGCGGCTCACTGGCCCGATACTG556760.11926713733014485No Hit
TGTGGATAGGGGTGAAAGGCTAATCAAACTCGGTGATAGCTGGTTCTCCC549120.1176305238356368No Hit
AAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGCTAATCAAACTCG540110.1157004338375324No Hit
CTCCGAGAGCCACCCCAATGCCACTTTCAGCGCCGTGGGTGAGGCCAGCA506650.10853275222415025No Hit
AGAAGGTGCGCCGGCGAGGGTTAAGGACTTGCTCCGTAAGCCCATGTCGG500830.10728601262493076No Hit
GAGAAGGTACGCTGCCGACGGTGATAGGATTTACTCCTTGAGCTATTGGC500260.10716390926212059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)