FastQCFastQC Report
Wed 27 Sep 2023
EGAF00004455797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46681761
Sequences flagged as poor quality0
Sequence length50-120
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCAACTTTGCTTTTAATGTGGCCAACCCACTCCAGCCCCTCCCCTGGA10814552.3166542496115343No Hit
AAAAACACGTTGTATTTGCAAGTGAACAGCCTGAAACCCGAGGATACAGC4060070.8697336846397032No Hit
AGCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAG3728220.7986459636773342No Hit
CTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAGT3381220.7243128638613269No Hit
GGATAGGAACCCTTGGTCTTCCGGCGGGGAAGTTTTTCACTCCCCTTATC3112930.6668407389344202No Hit
TACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGT2442710.5232686059122748No Hit
GGCATGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTA2251760.4823639793708725No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG1675170.35884893031349013No Hit
GCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGAT1642600.35187190131923257No Hit
ATAACTAGAAGTACAGGAATATTAACCTGTTTTCCATCGACTACGCTTTT1524550.3265836522319713No Hit
TGCAAGTCGAGCGGGCGTAGCAATACGTCAGCGGCAGACGGGTGAGTAAC1394940.2988190612603496No Hit
CGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGA1290180.27637774847439883No Hit
TCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAGTG967710.2072993775877478No Hit
ACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGC900290.19285690614799214No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA886340.18986858700553305No Hit
CTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACA883170.1891895209351678No Hit
AGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTG872880.18698523391180552No Hit
CAGCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACA843060.18059730008900052No Hit
CAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAA805320.17251277217241226No Hit
TAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAG774600.1659320435662228No Hit
GAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAG754710.16167127885342628No Hit
GGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGAGGACT738060.15810457536081382No Hit
TACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGGTCTAATGTGGAGG693750.14861264552551906No Hit
CTTGACATGTTTGGGACCGGTCGCAGAGACGTGACCTTCTCTTCGGAGCC644810.13812889363792424No Hit
TCAATGGCAGTTTCATAGTTAAGCTATGAGATTTCACCACTGACTGATTA629250.13479568604963296No Hit
ACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACG533660.11431873788994378No Hit
CAGACCCTGTCAGGACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCT530200.11357754905604353No Hit
TAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAATGAATTGAC512160.10971308473131508No Hit
CTCCGAGAGCCACCCCAATGCCACTTTCAGCGCCGTGGGTGAGGCCAGCA506840.10857345334508695No Hit
GAGTCAGACCCTGTCAGGACACAGCATAGACATGAGGGTCCCCGCTCAGC506600.10852204140285111No Hit
AGCTTAGGGTGCATCGCAAGATGCGCGGTAGCGGAGCGTTCTGTAAGCCT478030.1024018781125245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)