FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004455964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5070212
Sequences flagged as poor quality0
Sequence length75
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA558651.1018276947788377No Hit
CGAGCGACCGGCAAGGCGGAGGTCGACCCACGCCACACGTCGCACGAACGCCTGTCCGGGAGGGACCACCGGGCC106350.20975454280807193No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101630.2004452673773799No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA98290.19385777162769527Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)