FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004457266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15363480
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG15350.025.5810996
CCGTGCG24700.022.6419859
TAGTCCG31750.019.4882685
GTACTAG22900.018.5305521
GTATAGA45700.017.9171871
CTTACCG19550.017.650829
CGTGCGA13500.017.62827710-11
GTATAGG67100.016.8346751
TTGTACG15700.016.673889
AACCACG44300.016.3847477
CTACACT89300.016.1896864
CCTATAC64100.016.1500853
TATACTG46800.015.5094935
AGTCCGT43000.015.4964856
CCGATCG3902.1636515E-615.2552399
ACTGATC67200.015.1394628
GGACCGT13800.015.089426
GTGTAGA79100.015.0363381
CCACGAC41200.015.0182649
TCTATAC51150.014.8883973
Produced by FastQC (version 0.11.5)