FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004457345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508458
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGCAACGTGTAGATCT20800.40908000267475386Illumina Single End PCR Primer 1 (97% over 35bp)
CAAGCAGAAGACGGCATACGAGATAGTCCGGCTGGCTGACTATCTCGTAT5460.10738350070212288RNA PCR Primer, Index 37 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)