FastQCFastQC Report
Wed 8 May 2024
EGAF00004457352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497936
Total Bases75.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCATCATCTCGTAT34780.698483339224318TruSeq Adapter, Index 20 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)