FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004457535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909390
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGAGCCAGTGTAGATCT87140.45637612012213324Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCC6700.025.964605145
GCGTCGG13350.021.175255145
TCTCGGG6850.020.105196145
CGGTGGG12800.017.554846145
AGCCAGG10700.016.258211145
AAAGAGG37150.011.1214485145
GTAGGGG8901.0587846E-610.587626145
GTCTAGG2150.00809755510.11412145
CCCTGGT5800.00284940910.0441581
CTGAGTA6506.72835E-410.0368443
AGAGCCC7301.7660996E-49.929387145
CCCTCAG6050.0040400819.629111
TCGCCGG22100.09.183559145
GCCCCCC7350.00206978928.875656145
CCCTGAG7450.00224390728.7970651
CCCCCCC20259.276846E-118.23281145
AGAGCGG20358.8220986E-107.836165145
TAGGGCA12558.15971E-57.508357145
CCCACGC10850.00103973357.3826871
GTCGTGG11803.288042E-47.3713074145
Produced by FastQC (version 0.11.5)