FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004457584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169545
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTGTATCTCGTAT43140.3688613948159327TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT9251.8205017E-59.444551
GTTTAAT8104.7965703E-48.9878681
CTCATAA10004.3546426E-48.0081911
CAACTGG8250.0056458367.94200751
ACTGTAA12558.068857E-57.5144377145
GGTTGCA9800.00315463827.4287481
CTGTAGC10100.00438332377.1824985145
CTTCCGA11150.00139156627.1822341
GACTTCT10100.0044107927.1778927
CCAAAAA15652.1613108E-56.9778021
TCAGAAT13150.00112941666.61566933
GCTTGAA67100.06.2705026145
CTTGAAG13950.0020977356.2625151
AAGATTA13150.0081887626.06436354
GCACGGC14500.00337464046.00229362
CCGTCTT92350.05.8914375145
CTTTTAT20501.4295615E-45.68208651
CTTTCGG15800.0083852415.5292461
CTCTTTT25201.5319338E-55.489021
AAAAAAA337300.05.3337445145
Produced by FastQC (version 0.11.5)