FastQCFastQC Report
Sat 13 May 2023
EGAF00004457661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86809929
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2753950.31723905683645937No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2213190.2549466432578236No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1228630.14153104537154962TruSeq Adapter, Index 3 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA919520.10592336736043179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)