FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70122275
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC36791075.24670227827035TruSeq Adapter, Index 2 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3697170.5272461568025282No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2250570.3209493702250818No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1348260.19227271220165063No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAACTCGTATGC1294960.18467170382021975TruSeq Adapter, Index 2 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1129540.1610814823107208No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAGCTCGTATGC910030.1297775920704227TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAACCTCGTATGC871140.1242315655046845TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCCAACAATCTCGTATGC775170.11054547217699369TruSeq Adapter, Index 23 (97% over 36bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT772640.11018467384294077No Hit
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCCAACAATCTCGTATGC715090.10197758130351589TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCCAACAATCTCGTATGC702540.10018785043696884TruSeq Adapter, Index 23 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)