FastQCFastQC Report
Sat 13 May 2023
EGAF00004457665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83400327
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC21978572.6353098111953446TruSeq Adapter, Index 7 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3993620.4788494414416385No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATACCTCGTATGC3274290.39259918009673994TruSeq Adapter, Index 7 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2413260.2893585776947853No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1565710.18773427590997335No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1135590.13616133663360816No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1089630.13065056687367663No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATAACTCGTATGC985770.1181973782908549TruSeq Adapter, Index 7 (97% over 37bp)
CGGGAGGTGAGGGGCGTTTTTGTTCGGTCGTTTTTATTGGGAAGTGAGGA854940.1025103894376817No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)