FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74720786
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC23316403.120470386914827TruSeq Adapter, Index 2 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4020100.5380162890684795No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2541650.3401530064204625No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1695520.22691410125155803No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1131240.15139562370235238No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1088040.14561409993733204No Hit
CGGGAGGTGAGGGGCGTTTTTGTTCGGTCGTTTTTATTGGGAAGTGAGGA816400.10926009263339388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)