FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77775458
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC67559198.68644065072558TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3604880.4634983955992904No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2568060.3301889909796481No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAACTCGTATGC1995590.2565835099293147TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCACCTCGTATGC1801960.2316874816732034TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAGCTCGTATGC1735290.2231153688609587TruSeq Adapter, Index 1 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1400690.18009408572045954No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGTATCTCGTATGC1312720.1687833197973582TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACATGAGCATCTCGTATGC1232620.15848444119737617TruSeq Adapter, Index 15 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1231590.15835200867605306No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGAGCATCTCGTATGC1146470.14740768225370013TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATGAGCATCTCGTATGC1124810.14462274204801212TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGAGCATCTCGTATGC1081350.1390348610997572TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACATGAGCATCTCGTATGC1073110.1379754009291723TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACATGAGCATCTCGTATGC1042350.13402042582635773TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATATGAGCATCTCGTATGC1025000.13178964500601206TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC857940.11030986149898339TruSeq Adapter, Index 15 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCC801550.10305950239470144TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)