FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68349904
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC16425362.4031284667203043TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2664610.3898483895456532No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2170720.3175893268262674No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCACCTCGTATGC1088450.15924674890545565TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA991970.14513114751412087No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT939520.13745739862341286No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAACTCGTATGC718820.10516766782876535TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)