FastQCFastQC Report
Tue 27 Sep 2022
EGAF00004457671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72169721
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC69832049.676085625992652TruSeq Adapter, Index 3 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4301570.5960352818878155No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2698920.37396846802275985No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGAACTCGTATGC2102370.2913091488880773TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGAGCTCGTATGC1818070.2519158969729147TruSeq Adapter, Index 3 (97% over 36bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1763340.24433238421414988No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGACCTCGTATGC1692700.23454434582059697TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTAATCGATCTCGTATGC1437500.1991832558144433TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTAATCGATCTCGTATGC1261130.17474502915149137TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTAATCGATCTCGTATGC1213070.16808572669970556TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTAATCGATCTCGTATGC1201340.1664603913322597TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTAATCGATCTCGTATGC1180660.16359492369383TruSeq Adapter, Index 10 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1162690.1611049597933183No Hit
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTAATCGATCTCGTATGC1127280.15619846999269957TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTAATCGATCTCGTATGC1124450.15580633878299183TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATTGATCTCGTATGC1051170.14565249601006494TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC1009690.13990493326141584TruSeq Adapter, Index 10 (97% over 35bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT971640.13463263908142306No Hit
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC770660.10678439507892791TruSeq Adapter, Index 10 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)