FastQCFastQC Report
Sat 1 Apr 2023
EGAF00004457673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58539673
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC46765417.9886694959843725TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2951470.5041828641577824No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2094590.3578069183953248No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCACCTCGTATGC1838130.31399731255758806TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAACTCGTATGC1303610.2226882955085861TruSeq Adapter, Index 1 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1196310.2043588456669377No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAGCTCGTATGC928030.1585300963331312TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA884240.1510496992355936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGTATCTCGTATGC838960.1433147738970117TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACATGAGCATCTCGTATGC819730.14002982216863427TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATGAGCATCTCGTATGC751570.12838643632327773TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGAGCATCTCGTATGC736030.1257318263462114TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATATGAGCATCTCGTATGC735270.12560199986084652TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACATGAGCATCTCGTATGC732130.12506561148710207TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACATGAGCATCTCGTATGC691300.11809085438519618TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGAGCATCTCGTATGC685450.11709153209653221TruSeq Adapter, Index 15 (97% over 36bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT618320.10562409530370967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)