FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80600687
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC27200383.37470820813227TruSeq Adapter, Index 3 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA5613580.6964680090133724No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3877710.48110135835442697No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2462670.3055395793338585No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1446710.17949102592636712No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1379870.17119829263986297No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT980040.12159201571073457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)