FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76439249
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC47526186.217510064757438TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGACCTCGTATGC4811320.6294305691046232TruSeq Adapter, Index 3 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4023790.5264036542274244No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3034990.39704602539985706No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGAACTCGTATGC2235460.2924492363864014TruSeq Adapter, Index 3 (97% over 37bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1767860.23127647421025813TruSeq Adapter, Index 18 (97% over 35bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1480130.19363481710815866No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGAGCTCGTATGC1403270.18357977326543332TruSeq Adapter, Index 3 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1219060.15948089704544324No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC824490.10786212721686995TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTAGAGATCTCGTATGC782810.10240943105027105TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTAGAGATCTCGTATGC779980.10203920239980378TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTAGAGATCTCGTATGC774060.1012647311592504TruSeq Adapter, Index 18 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)