FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69243010
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC49840737.197943879100577TruSeq Adapter, Index 10 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3784250.5465172585651606No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2465650.35608648439748647No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1409440.20354978791361036No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAGCTCGTATGC1300310.1877893523115185TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAACTCGTATGC1295530.1870990299237425TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGACCTCGTATGC1091740.1576679003411319TruSeq Adapter, Index 10 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1037790.1498765001694756No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC968710.13990004189592567No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT968180.1398234998738501No Hit
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTT926650.13382578255913485No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT923000.13329865353918033No Hit
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTCGAAGATCTCGTATGC854460.12340018147680178TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTCGAAGATCTCGTATGC850800.1228716082677515TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC790980.11423246909688069TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAAGATCTCGTATGC786210.11354359089820042TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTCGAAGATCTCGTATGC771090.11135997698540256TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTCGAAGATCTCGTATGC752260.10864056891807562TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTCGAAGATCTCGTATGC715560.10334039493661526TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTCGAAGATCTCGTATGC704980.10181244287329509TruSeq Adapter, Index 3 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)