FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004457678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57505510
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC583566910.148017120446372TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2519940.43820844298224637No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1846640.3211240105513367No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAACCTCGTATGC1452410.2525688407945604TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAAACTCGTATGC1357860.23612693809688845TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCAATCTCGTATGC1113200.19358144984715378TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTCAATCTCGTATGC1097140.19078867398967508TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCACTCAATCTCGTATGC1060950.18449536400946623TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCACTCAATCTCGTATGC1023420.17796903288050137TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTAATCTCGTATGC1019790.17733778902230413TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCACTCAATCTCGTATGC1019490.1772856201084035TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCACTCAATCTCGTATGC998510.173637274062955TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCACTCAATCTCGTATGC964460.1677161023352371TruSeq Adapter, Index 7 (97% over 35bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT953420.16579628630369508No Hit
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCACTCAATCTCGTATGC917810.15960383622369403TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCACTCAATCTCGTATGC906640.1576614136627951TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAAGCTCGTATGC857830.14917353137116773TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCC801940.13945446271148626TruSeq Adapter, Index 7 (97% over 34bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA798880.13892233978970014No Hit
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC761750.13246556721260278TruSeq Adapter, Index 7 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC640220.11133194019147036TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)